An experiment on brain heart agar and thioglyollate broth to determine the used bacterias oxygen req

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Bacteriocin is a protein which is secreted by bacteria that kills or inhbits the strain of certain competing bacteria strain.

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Usually bacteriocin will only kills the closely-related bacteria strains. There is three main known ways of how bacteriocins inhibit the growth of pathogenic bacteria strains. The bacteriocin will cause the destruction of the membrane potential by forming the pores on the pathogenic bacteria.

It will inhibits the nucleolytic activity of the pathogenic bacteria strains which breaks down the DNA chromosomes as well as RNA. The third way is the bacteriocin will inhibits the protein synthesis of the pathogenic bacterias but does not kills them. Part 1 Determination of bacteriocin activity via agar diffusion test: This might be due to two reasons: Besides that, we can observe that the inhibition zone between Lactobacillus plantarum and three pathogenic bacteria strain is the biggest.

Part 2 Determination of bacteriocin activity via optical density: Optical density, measured in a spectrophotometer, can be used as a measure of the concentration of bacteria in a suspension. As visible light passes through a cell suspension the light is scattered. Greater scatter indicates that more bacteria or other material is present.

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The amount of light scatter can be measured in a spectrophotometer. Typically, when working with a particular type of cell, you would determine the optical density at a particular wavelength that correlates with the different phases of bacterial growth. Generally we will want to use cells that are in their mid-log phase of growth.

It indicated that the LAB has stronger antimicrobial effect on the pathogenic bacteria compare to a more diluted solution. From the results of our experiment, the graph shows us when the serial dilution is increasing, the optical density is decreasing.

An experiment on brain heart agar and thioglyollate broth to determine the used bacterias oxygen req

The outcome is totally different from what we expect. This might be caused by the using of distilled water during the process of serial dilution.

An experiment on brain heart agar and thioglyollate broth to determine the used bacterias oxygen req

As we know, the distilled water is colourless. So, the results obtained is wrong. Then the result will not be affected.

Conclusions LAB is a useful bacteria used to produce bacteriocin that can inhibit the growth of bacteria.Pathogen translocation and histopathological lesions in an experimental model of Salmonella Dublin infection in calves receiving lactic acid bacteria and lactose supplements.

Argentina) and stored at in brain heart infusion (BHI) broth (Britania, Argentina) with glycerol (35% v/v). The biochemical profile of the strain was determined. Examples of standard general purpose media that will support the growth of a wide variety of bacteria include nutrient agar, tryptic soy agar, and brain heart infusion agar.

A medium may be enriched, by the addition of blood or serum. Examples of enriched media include sheep blood agar and chocolate (heated blood) agar. An Experiment on Brain, Heart Agar and Thioglyollate Broth to Determine the Used Bacteria's Oxygen Requirements Using 8 Different Bacteria.

Carbon Dioxide. While some chemoautotrophs are aerobic, using oxygen as the ultimate electron acceptor and deriving energy from the respiration of various inorganic electron donors, other microorganisms engage in anaerobic respiration, using an inorganic terminal electron acceptor other than oxygen.

In this experiment, all these aspects are studied.

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The differences between self made and commercial agar are compared and contrasted based on the morphology of the colonies. (with 15 g/ L of Agar), 1 L of own-prepared Nutrient Broth Agar (NBA), mL of Brain-Heart Infusion (BHI) broth and mL of Trypticase Soy Agar with % Yeast.

Techniques similar to those used by his group will likely be used to determine the properties of other biofilm signaling molecules. Development Once colonization has begun, the biofilm grows through a combination of cell division and recruitment.

Use of Liquid Nutrient Broth Media for Growing Bacteria